General Information

  • ID:  hor006334
  • Uniprot ID:  Q9TWD3
  • Protein name:  Peptide K12=BRADYKININ-potentiating peptide/BPP
  • Gene name:  NA
  • Organism:  Buthus occitanus (Common European scorpion)
  • Family:  Non-disulfide-bridged peptide (NDBP) superfamily, Scorpion BPP (group 1) family
  • Source:  animal
  • Expression:  Expressed by the venom gland.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:   Buthus (genus), Buthidae (family), Buthoidea (superfamily), Buthida (parvorder), Scorpiones (order), Arachnida (class), Chelicerata (subphylum), Arthropoda (phylum), Panarthropoda , Ecdysozoa , Protostomia , Bilateria , Eumetazoa , Metazoa (kingdom), Opisthokonta , Eukaryota (superkingdom),cellular organisms
  • GO MF:  GO:0004857 enzyme inhibitor activity; GO:0008191 metalloendopeptidase inhibitor activity; GO:0030414 peptidase inhibitor activity
  • GO BP:  GO:0008217 regulation of blood pressure
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  LRDYANRVINGGPVEAAGPPA
  • Length:  21
  • Propeptide:  LRDYANRVINGGPVEAAGPPA
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Inhibits angiotensin-converting enzyme (ACE), but does not serve as substrate for the enzyme. Potentiate bradykinin (BK) on the isolated guinea pig ileum as well as the isolated rat uterus for contraction. Also potentiates in vivo the depressor effect of BK on arterial blood pressure in the normotensive anesthetized rat. Intracerebroventricular injection into mice does not show toxic activity.
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q9TWD3-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-Q9TWD3-F1.pdbhor006334_AF2.pdbhor006334_ESM.pdb

Physical Information

Mass: 249431 Formula: C93H149N29O29
Absent amino acids: CFHKMQSTW Common amino acids: A
pI: 6.49 Basic residues: 2
Polar residues: 6 Hydrophobic residues: 8
Hydrophobicity: -30.48 Boman Index: -3081
Half-Life: 5.5 hour Half-Life Yeast: 3 min
Half-Life E.Coli: 2 min Aliphatic Index 83.81
Instability Index: 5620.48 Extinction Coefficient cystines: 1490
Absorbance 280nm: 74.5

Literature

  • PubMed ID:  8745044
  • Title:  A bradykinin-potentiating peptide (peptide K12) isolated from the venom of Egyptian scorpion Buthus occitanus.
  • PubMed ID:  24184590
  • Title:  Scorpion venom peptides with no disulfide bridges: a review.