HORDB

General Information

ID: hor005896
Uniprot ID: Q9VVF7
Protein name: Drostatin-B1
Gene name: Mip
Organism: Drosophila melanogaster (Fruit fly)
Family: NA
Source: Animal
Expression: Up-regulated by sleep deprivation. |Expressed throughout development, with higher expression in larvae than in embryos (at protein level) . |In larvae, strongly expressed in the midgut region before and in between the copper cells, and in a group of cells
Disease: NA
Comments: NA
Taxonomy: melanogaster subgroup, melanogaster group, Sophophora (subgenus), Drosophila (genus), Drosophilini (tribe), Drosophilinae (subfamily), Drosophilidae (family), Ephydroidea (superfamily), Acalyptratae, Schizophora, Cyclorrhapha, Eremoneura, Muscomorpha (infraorder), Brachycera (suborder), Diptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
GO MF: GO:0001664 G protein-coupled receptor binding; GO:0005102 signaling receptor binding; GO:0005184 neuropeptide hormone activity
GO BP: GO:0007186 G protein-coupled receptor signaling pathway; GO:0007218 neuropeptide signaling pathway; GO:0045968 negative regulation of juvenile hormone biosynthetic process; GO:1903999 negative regulation of eating behavior
GO CC: GO:0005576 extracellular region; GO:0005615 extracellular space

Sequence Information

Sequence: AWQSLQSSW
Length: 9(66-74)
Propeptide: MAHTKTRRTYGFLMVLLILGSACGNLVASGSAGSPPSNEPGGGGLSEQVVLDQLSESDLYGNNKRAWQSLQSSWGKRSSSGDVSDPDIYMTGHFVPLVITDGTNTIDWDTFERLASGQSAQQQQQQPLQQQSQSGEDFDDLAGEPDVEKRAWKSMNVAWGKRRQAQGWNKFRGAWGKREPTWNNLKGMWGKRDQWQKLHGGWGKRSQLPSN
Signal peptide: MAHTKTRRTYGFLMVLLILGSACG
Modification: T9 Tryptophan amide
Glycosylation: NA
Mutagenesis: 2-2W->A: Complete loss of activity; 9-9W->A: Complete loss of activity

Activity

Function: Ligand for the sex peptide receptor (SPR) (PubMed:20308537, PubMed:20458515, PubMed:25333796). Stabilizes sleep and maintains sleep homeostasis to inhibit the activity of wake-promoting circuits, such as those that involve the pigment dispersing factor (p
Mechanism: All five Drostatin-B peptides activate SPR with comparable efficiency, however Drostatin-B4 is a slightly more potent ligand.
Cross BBB: NA
Target: NA
Target Unid: NA
IC50: NA
EC50: NA
ED50: NA
kd: NA
Half life: NA

Structure

Disulfide bond: NA
Structure ID: AF-Q9VVF7-F1(AlphaFold_DB_ID)

Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)

hor005896_AF2.pdb

hor005896_ESM.pdb

Physical Information

Mass:	123505	Formula:	C50H69N13O15
Absent amino acids:	CDEFGHIKMNPRTVY	Common amino acids:	S
pI:	6.11	Basic residues:	0
Polar residues:	3	Hydrophobic residues:	4
Hydrophobicity:	-62.22	Boman Index:	-989
Half-Life:	4.4 hour	Half-Life Yeast:	>20 hour
Half-Life E.Coli:	>10 hour	Aliphatic Index	54.44
Instability Index:	16415.56	Extinction Coefficient cystines:	11000
Absorbance 280nm:	1375

Literature

PubMed ID: 11181081
Title: Molecular cloning, genomic organization, and expression of a B-type (cricket-type) allatostatin preprohormone from Drosophila melanogaster.
PubMed ID: 10731132
Title: The genome sequence of Drosophila melanogaster.
PubMed ID: 12537572
Title: Annotation of the Drosophila melanogaster euchromatic genome: a s
PubMed ID: 12171930
Title:
PubMed ID: 21214272
Title:
PubMed ID: 19319573
Title:
PubMed ID: 20458515
Title:
PubMed ID: 20308537
Title:
PubMed ID: 21174124
Title:
PubMed ID: 25333796
Title: