General Information

  • ID:  hor005823
  • Uniprot ID:  P85160
  • Protein name:  Bradykinin-potentiating peptide 7a
  • Gene name:  NA
  • Organism:  Bothrops jararaca (Jararaca) (Bothrops jajaraca)
  • Family:  Bradykinin-potentiating peptide family
  • Source:  animal
  • Expression:  Expressed by the venom gland.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:   Bothrops (genus), Crotalinae (subfamily), Viperidae (family), Colubroidea (superfamily), Serpentes (infraorder), Toxicofera , Episquamata , Unidentata , Bifurcata , Squamata (order), Lepidosauria (class), Sauria , Sauropsida , Amniota , Tetrapoda , Dipnotetrapodomorpha , Sarcopterygii (superclass), Euteleostomi , Teleostomi , Gnathostomata , Vertebrata , Craniata (subphylum), Chordata (phylum), Deuterostomia , Bilateria , Eumetazoa , Metazoa (kingdom), Opisthokonta , Eukaryota (superkingdom),cellular organisms
  • GO MF:  GO:0090729 toxin activity
  • GO BP:  GO:0008217 regulation of blood pressure; GO:0035821 modulation of process of another organism
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  QDGPIPP
  • Length:  7
  • Propeptide:  QDGPIPP
  • Signal peptide:  NA
  • Modification:  T1 Pyrrolidone carboxylic acid
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  This peptide shows potent and long-lasting antihypertensive activity as well as a reduction of the heart rate. These effects may be explained by the agonistic activities of this peptide for the muscarinic acetylcholine receptor M1 (CHRM1) (EC(50)=0.25-0.31 uM), since these types of G-protein coupled receptor have been demonstrated to be active in the blood pressure control.
  • Mechanism:  Negative results: weakly inhibits angiotensin-converting enzyme (ACE) with no clear preference for either C- or N-domains. It does not potentiate bradykinin, and does not affect angiotensin-1 pressor effects. It does not bind to type-1 angiotensin-2 receptor (AGTR1) (PubMed:17475904). It does not bind to the muscarinic acetylcholine receptor M3 (CHRM3) (PubMed:20814884).
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor005823_AF2.pdbhor005823_ESM.pdb

Physical Information

Mass: 83000 Formula: C32H50N8O11
Absent amino acids: ACEFHKLMNRSTVWY Common amino acids: P
pI: 3.75 Basic residues: 0
Polar residues: 1 Hydrophobic residues: 1
Hydrophobicity: -110 Boman Index: -840
Half-Life: 0.8 hour Half-Life Yeast: 10 min
Half-Life E.Coli: >10 hour Aliphatic Index 55.71
Instability Index: 5948.57 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  15245866
  • Title:  Identification of five new bradykinin potentiating peptides (BPPs) from Bothrops jararaca crude venom by using electrospray ionization tandem mass spectrometry after a two-step liquid chromatography.
  • PubMed ID:  17475904
  • Title:  Do the cardiovascular effects of angiotensin-converting enzyme (ACE) I involve ACE-independent mechanisms? new insights from proline-rich peptides of Bothrops jararaca.
  • PubMed ID:  20814884
  • Title:  The snake venom peptide Bj-PRO-7a is a M1 muscarinic acetylcholine receptor agonist.