General Information

  • ID:  hor005158
  • Uniprot ID:  P34963
  • Protein name:  Neuromedin-U-25
  • Gene name:  NMU
  • Organism:  Gallus gallus (Chicken)
  • Family:  NmU family
  • Source:  animal
  • Expression:  widely expressed in chicken tissues with abundant expression noted in the gastrointestinal tract
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Gallus (genus), Phasianinae (subfamily), Phasianidae (family), Galliformes (order), Galloanserae (superorder), Neognathae (infraclass), Aves (class), Coelurosauria, Theropoda, Saurischia, Dinosauria, Archosauria, Archelosauria, Sauria, Sauropsida, Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity
  • GO BP:  GO:0006940 regulation of smooth muscle contraction; GO:0007165 signal transduction
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  YKVDEDLQGAGGIQSRGYFFFRPRN
  • Length:  25
  • Propeptide:  YKVDEDLQGAGGIQSRGYFFFRPRN
  • Signal peptide:  NA
  • Modification:  T25 Asparagine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  NMU may be a local factor that regulates testicular function and act as an autocrine/paracrine factor to regulate pituitary functions.
  • Mechanism:  It can activates NMUR1, which can stimulate multiple downstream signaling pathways, including calcium mobilization and MPAK / ERK signaling.
  • Cross BBB:  NO
  • Target:  NMUR1, NMUR2
  • Target Unid:  E1BWP2, E1BX29
  • IC50: NA
  • EC50: 20.8 nM
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-P34963-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-P34963-F1.pdbhor005158_AF2.pdbhor005158_ESM.pdb

Physical Information

Mass: 334948 Formula: C132H194N38O38
Absent amino acids: CHMTW Common amino acids: G
pI: 9.14 Basic residues: 4
Polar residues: 8 Hydrophobic residues: 7
Hydrophobicity: -89.2 Boman Index: -6757
Half-Life: 2.8 hour Half-Life Yeast: 10 min
Half-Life E.Coli: 2 min Aliphatic Index 46.8
Instability Index: 4719.2 Extinction Coefficient cystines: 2980
Absorbance 280nm: 124.17

Literature

  • PubMed ID:  1455013
  • Title:  The purification and sequence analysis of an avian neuromedin U.
  • PubMed ID:  1788145
  • Title:   Primary structure and pharmacological activity of a nonapeptide related to neuromedin U isolated from chicken intestine.
  • PubMed ID:  29288685
  • Title:  Characterization of neuromedin U (NMU), neuromedin S (NMS) and their receptors (NMUR1, NMUR2) in chickens.