General Information

  • ID:  hor005048
  • Uniprot ID:  P84215
  • Protein name:  Tryptophyllin-13
  • Gene name:  NA
  • Organism:  Bombina variegata (Yellow-bellied toad)
  • Family:  Frog skin active peptide (FSAP) family, Tryptophillin subfamily
  • Source:  animal
  • Expression:  Expressed by the skin glands.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Bombina (genus), Bombinatoridae (family), Anura (order), Batrachia (superorder), Amphibia (class), Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity
  • GO BP:  GO:0006952 defense response; GO:0007165 signal transduction; GO:0050796 regulation of insulin secretion
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  GKPFYPPPIYPEDM
  • Length:  14
  • Propeptide:  GKPFYPPPIYPEDM
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Possesses insulin-releasing activity
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor005048_AF2.pdbhor005048_ESM.pdb

Physical Information

Mass: 188295 Formula: C80H111N15O21S
Absent amino acids: ACHLNQRSTVW Common amino acids: P
pI: 4.18 Basic residues: 1
Polar residues: 3 Hydrophobic residues: 2
Hydrophobicity: -90.71 Boman Index: -1017
Half-Life: 30 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 27.86
Instability Index: 10619.29 Extinction Coefficient cystines: 2980
Absorbance 280nm: 229.23

Literature

  • PubMed ID:  15134346
  • Title:  Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures.