General Information

  • ID:  hor004826
  • Uniprot ID:  P41495
  • Protein name:  Trypsin-modulating oostatic factor
  • Gene name:  N/A
  • Organism:  Sarcophaga bullata (Grey flesh fly) (Neobellieria bullata)
  • Family:  NA
  • Source:  Animal
  • Expression:  Synthesized and released from follicular epithelium after a blood meal.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Neobellieria (subgenus), Sarcophaga (genus), Sarcophaginae (subfamily), Sarcophagidae (family), Oestroidea (superfamily), Calyptratae, Schizophora, Cyclorrhapha, Eremoneura, Muscomorpha (infraorder), Brachycera (suborder), Diptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity; GO:0005515 protein binding
  • GO BP:  GO:0007165 signal transduction
  • GO CC:  NA

Sequence Information

  • Sequence:  NPTNLH
  • Length:  6(1-6)
  • Propeptide:  NPTNLH
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Has an oostatic activity. Inhibits trypsin biosynthesis in the midgut which indirectly reduces the vitellogenin concentration in the hemolymph resulting in inhibition of oocyte development.
  • Mechanism:  NA
  • Cross BBB:  NO
  • Target:  NA
  • Target Unid:   NA
  • IC50:  NA
  • EC50:  NA
  • ED50:  NA
  • Kd:  NA
  • Half life:  NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004826_AF2.pdbhor004826_ESM.pdb

Physical Information

Mass: 78411 Formula: C29H46N10O10
Absent amino acids: ACDEFGIKMQRSVWY Common amino acids: N
pI: 7.55 Basic residues: 1
Polar residues: 3 Hydrophobic residues: 1
Hydrophobicity: -145 Boman Index: -1559
Half-Life / Aliphatic Index: 1.4 hour Aliphatic Index: 65
Instability Index: -2151.67 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  8159807
  • Title:  Sequencing and characterization of trypsin modulating oostatic factor (TMOF) from the ovaries of the grey fleshfly, Neobellieria (Sarcophaga) bullata.