General Information

  • ID:  hor004800
  • Uniprot ID:  P12797
  • Protein name:  Thr6-BK
  • Gene name:  NA
  • Organism:  Megascolia flavifrons (Garden dagger wasp) (Solitary wasp)
  • Family:  Bradykinin-related peptide family
  • Source:  animal
  • Expression:  Expressed by the venom gland.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Megascolia (genus), Scoliidae (family), Scolioidea (superfamily), Aculeata (infraorder), Apocrita (suborder), Hymenoptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0003674 molecular_function; GO:0090729 toxin activity
  • GO BP:  GO:0035821 modulation of process of another organism; GO:0045776 negative regulation of blood pressure; GO:0045987 positive regulation of smooth muscle contraction
  • GO CC:  GO:0005576 extracellular region; GO:0005615 extracellular space

Sequence Information

  • Sequence:  RPPGFTPFR
  • Length:  9
  • Propeptide:  RPPGFTPFRKA
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Inhibits angiotensin-converting enzyme (ACE) with a Ki of 1.6 ¦ÌM;caused contraction in guinea pig ileum smooth muscle preparations and was completely blocked by a B2 receptor antagonist;central anti-nociceptive effects were assessed after intracerebroventricular injection in rat
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: 26.7+/-7.7 nM
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004800_AF2.pdbhor004800_ESM.pdb

Physical Information

Mass: 121710 Formula: C51H75N15O11
Absent amino acids: ACDEHIKLMNQSVWY Common amino acids: P
pI: 12.5 Basic residues: 2
Polar residues: 2 Hydrophobic residues: 2
Hydrophobicity: -103.33 Boman Index: -2551
Half-Life: 1 hour Half-Life Yeast: 2 min
Half-Life E.Coli: 2 min Aliphatic Index 0
Instability Index: 7308.89 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  19716363
  • Title:  Bradykinin-related peptides in the venom of the solitary wasp Cyphononyx fulvognathus.