General Information

  • ID:  hor004797
  • Uniprot ID:  P0DM72
  • Protein name:  nephilakin-I
  • Gene name:  NA
  • Organism:  Trichonephila clavipes (Golden silk orbweaver) (Nephila clavipes)
  • Family:  Bradykinin-related peptide family
  • Source:  animal
  • Expression:  venom
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Trichonephila (genus), Nephilidae (family), Araneoidea (superfamily), Orbiculariae, Entelegynae, Araneomorphae (suborder), Araneae (order), Arachnida (class), Chelicerata (subphylum), Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0090729 toxin activity
  • GO BP:  GO:0035821 modulation of process of another organism
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  GPNPGFSPFR
  • Length:  10
  • Propeptide:  GPNPGFSPFR
  • Signal peptide:  NA
  • Modification:  T10 Arginine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Cause constriction on isolated rat ileum preparations and relaxation on rat duodenum muscle preparations at amounts higher
  • Mechanism:  Has been extracted from the spider web.
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: 98pmoles/mg
  • EC50: NA
  • ED50: Constriction:7.0*10(-6)M;Relaxation:10*10(-6)M
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004797_AF2.pdbhor004797_ESM.pdb

Physical Information

Mass: 123588 Formula: C50H70N14O13
Absent amino acids: ACDEHIKLMQTVWY Common amino acids: P
pI: 10.55 Basic residues: 1
Polar residues: 4 Hydrophobic residues: 2
Hydrophobicity: -88 Boman Index: -1712
Half-Life: 30 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 0
Instability Index: 6932 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  16202476
  • Title:  Multiple bradykinin-related peptides from the capture web of the spider Nephila clavipes (Araneae, Tetragnatidae)