General Information

  • ID:  hor004781
  • Uniprot ID:  A0A1A7UPA5
  • Protein name:  RAP-L1, T6-BK
  • Gene name:  Kng1
  • Organism:  Odorrana livida (Green mountain frog) (Polypedates lividus)
  • Family:  Bradykinin-related peptide family
  • Source:  animal
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Odorrana (genus), Ranidae (family), Ranoidea (superfamily), Neobatrachia (suborder), Anura (order), Batrachia (superorder), Amphibia (class), Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  NA
  • GO BP:  GO:0050829 defense response to Gram-negative bacterium; GO:0050830 defense response to Gram-positive bacterium
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  RAPLPPGFTPFR
  • Length:  12
  • Propeptide:  MFTLKKSLLLLFFLGAINLSLCEQERDADEEENEREAKVETVKRAPLPPGFTPFRVAPEIV
  • Signal peptide:  MFTLKKSLLLLFFLGAINLSLC
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  This peptide exhibited a dose-dependent contractile property on rat bladder and rat ileum, and increased the contraction frequency on rat uterus ex vivo smooth muscle preparations; it also showed vasorelaxant activity on rat tail artery smooth muscle.RAP-L1, T6-BK -induced relaxation of the arterial smooth muscle was very likely to be modulated by B2 receptors.s
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: urinary bladder:3.54+/- 0.83*10(-6)M;ileum:70.95+/-0.98 nM;terus smooth muscle preparations:6.82+/-0.75*10(-6)M;tail artery:1.408+/-0.15 nM
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-A0A1A7UPA5-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004781_AF2.pdbhor004781_ESM.pdb

Physical Information

Mass: 155197 Formula: C65H98N18O14
Absent amino acids: CDEHIKMNQSVWY Common amino acids: P
pI: 12.5 Basic residues: 2
Polar residues: 2 Hydrophobic residues: 4
Hydrophobicity: -44.17 Boman Index: -1878
Half-Life: 1 hour Half-Life Yeast: 2 min
Half-Life E.Coli: 2 min Aliphatic Index 40.83
Instability Index: 7336.67 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  27690099
  • Title:  Ex Vivo Smooth Muscle Pharmacological Effects of a Novel Bradykinin-Related Peptide, and Its Analogue, From Chinese Large Odorous Frog, Odorrana Livida Skin Secretions