General Information

  • ID:  hor004712
  • Uniprot ID:  O65684
  • Protein name:  Protein CASPARIAN STRIP INTEGRITY FACTOR 2
  • Gene name:  CIF2
  • Organism:  Arabidopsis thaliana (Mouse-ear cress)
  • Family:  NA
  • Source:  Plant
  • Expression:  Induced by excess iron and further synergistically regulated by lowering the medium pH. |Confined to the root stele in the elongation and differentiation zones of both primary and lateral roots. Disappears at the sites of lateral root initiation. In the r
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Arabidopsis (genus), Camelineae (tribe), Brassicaceae (family), Brassicales (order), malvids, rosids, Pentapetalae, Gunneridae, eudicotyledons, Mesangiospermae, Magnoliopsida (class), Spermatophyta, Euphyllophyta, Tracheophyta, Embryophyta, Streptophytina (subphylum), Streptophyta (phylum), Viridiplantae (kingdom), Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity; GO:0005515 protein binding
  • GO BP:  GO:0007165 signal transduction; GO:0009860 pollen tube growth; GO:0071555 cell wall organization; GO:0160073 Casparian strip assembly; GO:2000067 regulation of root morphogenesis
  • GO CC:  GO:0048226 Casparian strip

Sequence Information

  • Sequence:  GRSSILIYSQEDDHPEVVERRIHEHERILRMNSRDYGHSSPKPKLVRPPFKLIPN
  • Length:  55(29-83)
  • Propeptide:  MGLLPLVKKLGFIIFLLVSASAFALCSAGRSSILIYSQEDDHPEVVERRIHEHERILRMNSRDYGHSSPKPKLVRPPFKLIPN
  • Signal peptide:  MGLLPLVKKLGFIIFLLVSASAFALCSA
  • Modification:  T36 Sulfotyrosine;T41 Hydroxyproline;T43 Hydroxyproline
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Peptide hormone required for contiguous Casparian strip diffusion barrier formation in roots via the regulation of CASPs protein expression and distribution in a GSO1-GSO2 signaling pathway. The Casparian strip is required for ion homeostasis (e.g. iron a
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:   NA
  • IC50:  NA
  • EC50:  NA
  • ED50:  NA
  • Kd:  NA
  • Half life:  NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-O65684-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004712_AF2.pdbhor004712_ESM.pdb

Physical Information

Mass: 749475 Formula: C287H460N90O83S
Absent amino acids: ACTW Common amino acids: RPS
pI: 9.86 Basic residues: 14
Polar residues: 12 Hydrophobic residues: 13
Hydrophobicity: -104.18 Boman Index: -17583
Half-Life / Aliphatic Index: 30 hour Aliphatic Index: 79.64
Instability Index: 7232 Extinction Coefficient cystines: 2980
Absorbance 280nm: 55.19

Literature

  • PubMed ID:  28104889
  • Title:  A peptide hormone required for Casparian strip diffusion barrier formation in Arabidopsis roots.