General Information

  • ID:  hor004617
  • Uniprot ID:  A1EC31
  • Protein name:  TDIFp
  • Gene name:  TDIF
  • Organism:  Zinnia violacea (Garden zinnia) (Zinnia elegans)
  • Family:  CLV3/ESR signal peptide family
  • Source:  Plant
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Zinnia (genus), Heliantheae (tribe), Heliantheae alliance, Asteroideae (subfamily), Asteraceae (family), Asterales (order), campanulids, asterids, Pentapetalae, Gunneridae, eudicotyledons, Mesangiospermae, Magnoliopsida (class), Spermatophyta, Euphyllophyta, Tracheophyta, Embryophyta, Streptophytina (subphylum), Streptophyta (phylum), Viridiplantae (kingdom), Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0033612 receptor serine/threonine kinase binding
  • GO BP:  GO:0010067 procambium histogenesis; GO:0010087 phloem or xylem histogenesis; GO:0010089 xylem development; GO:0030154 cell differentiation; GO:0045168 cell-cell signaling involved in cell fate commitment
  • GO CC:  GO:0005576 extracellular region; GO:0005886 plasma membrane; GO:0016020 membrane; GO:0048046 apoplast

Sequence Information

  • Sequence:  HEVPSGPNPISN
  • Length:  12(120-131)
  • Propeptide:  MDIDLLWSFGGWFFILFPETINYCMAKLRSTSQISHFTNPRSCSSLFFVALLIITILITMLQSSTSMEVTSLPTHQPTSSNSHDESSTSSTATTTTDLHPKRTHHQSHPKPTRSFEAGAHEVPSGPNPISNR
  • Signal peptide:  MDIDLLWSFGGWFFILFPETINYCMA
  • Modification:  T4 Hydroxyproline;T7 Hydroxyproline
  • Glycosylation:  T7 O-linked (Ara...) hydroxyproline
  • Mutagenesis:  1-1H->A: Impaired repression of tracheary element differentiation.; 2-2H->A: Impaired repression of tracheary element differentiation.; 3-3H->A: Impaired repression of tracheary element differentiation.; 5-5H->A: Impaired repression of tracheary element d

Activity

  • Function:  Extracellular signal peptide that regulates cell fate. Represses tracheary element differentiation but promotes the formation of procambial cells adjacent to phloem cells in the veins.
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:   NA
  • IC50:  NA
  • EC50:  NA
  • ED50:  NA
  • Kd:  NA
  • Half life:  NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-A1EC31-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004617_AF2.pdbhor004617_ESM.pdb

Physical Information

Mass: 144370 Formula: C53H82N16O19
Absent amino acids: ACDFKLMQRTWY Common amino acids: P
pI: 5.36 Basic residues: 1
Polar residues: 5 Hydrophobic residues: 2
Hydrophobicity: -98.33 Boman Index: -2165
Half-Life / Aliphatic Index: 3.5 hour Aliphatic Index: 56.67
Instability Index: 3886.67 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  16902140
  • Title:  Dodeca-CLE Peptides as Suppressors of Plant Stem Cell Differentiation