General Information

  • ID:  hor004557
  • Uniprot ID:  P0DM28
  • Protein name:  Conorfamide-Sr3
  • Gene name:  NA
  • Organism:  Conus spurius (Alphabet cone)
  • Family:  FARP (FMRFamide related peptide) family
  • Source:  Animal
  • Expression:  Expressed by the venom duct.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Lindaconus (subgenus), Conus (genus), Conidae (family), Conoidea (superfamily), Neogastropoda (order), Caenogastropoda (subclass), Gastropoda (class), Mollusca (phylum), Lophotrochozoa, Spiralia, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0015459 potassium channel regulator activity; GO:0090729 toxin activity; GO:0099106 ion channel regulator activity
  • GO BP:  GO:0035821 modulation of process of another organism
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  ATSGPMGWLPVFYRF
  • Length:  15(1-15)
  • Propeptide:  ATSGPMGWLPVFYRF
  • Signal peptide:  NA
  • Modification:  T15 Phenylalanine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Inhibits the Shaker voltage-gated potassium channels (Kv) from drosophila (Kd= 2.7 娄脤M
  • Mechanism:  Negative results: does not affect potassium channels from drosophila (Shab, Shaw, and Shal), as well as the human channel Kv10.1/KCNH1/EAG1 (PubMed:28774677). Does not show activity on human Kv1.4/KCNA4 and Kv1.5/KCNA5 (PubMed:32823677).
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004557_AF2.pdbhor004557_ESM.pdb

Physical Information

Mass: 197891 Formula: C84H117N19O19S
Absent amino acids: CDEHIKNQ Common amino acids: FGP
pI: 9.35 Basic residues: 1
Polar residues: 5 Hydrophobic residues: 6
Hydrophobicity: 34 Boman Index: 226
Half-Life: 4.4 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 52
Instability Index: 5690 Extinction Coefficient cystines: 6990
Absorbance 280nm: 499.29

Literature

  • PubMed ID:  28774677
  • Title:  Conorfamide-Sr3, a structurally novel specific inhibitor of the Shaker K+ channel.