General Information

  • ID:  hor004490
  • Uniprot ID:  P08612
  • Protein name:  Uperolein
  • Gene name:  NA
  • Organism:  Uperoleia rugosa (Wrinkled toadlet) (Pseudophryne rugosa)
  • Family:  Tachykinin family
  • Source:  Animal
  • Expression:  Expressed by the skin glands.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Uperoleia (genus), Myobatrachinae (subfamily), Myobatrachidae (family), Myobatrachoidea (superfamily), Neobatrachia (suborder), Anura (order), Batrachia (superorder), Amphibia (class), Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  NA
  • GO BP:  GO:0006952 defense response; GO:0007217 tachykinin receptor signaling pathway; GO:0007218 neuropeptide signaling pathway
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  QPDPNAFYGLM
  • Length:  11(1-11)
  • Propeptide:  QPDPNAFYGLM
  • Signal peptide:  NA
  • Modification:  T1 Pyrrolidone carboxylic acid;T11 Methionine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004490_AF2.pdbhor004490_ESM.pdb

Physical Information

Mass: 143096 Formula: C57H81N13O17S
Absent amino acids: CEHIKRSTVW Common amino acids: P
pI: 3.75 Basic residues: 0
Polar residues: 3 Hydrophobic residues: 3
Hydrophobicity: -46.36 Boman Index: -804
Half-Life: 0.8 hour Half-Life Yeast: 10 min
Half-Life E.Coli: >10 hour Aliphatic Index 44.55
Instability Index: 4166.36 Extinction Coefficient cystines: 1490
Absorbance 280nm: 149

Literature

  • PubMed ID:  1120493
  • Title:  Structure of uperolein, a physalaemin-like endecapeptide occurring in the skin of Uperoleia rugosa and Uperoleia marmorata.