General Information

  • ID:  hor003233
  • Uniprot ID:  P50745
  • Protein name:  Endomorphin-1
  • Gene name:  Sh2b3
  • Organism:  Rattus norvegicus (Rat)
  • Family:  SH2B adapter family
  • Source:  animal
  • Expression:  Preferentially expressed by lymphocytes in lymph node and spleen.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Rattus (genus), Murinae (subfamily), Muridae (family), Muroidea, Myomorpha (suborder), Rodentia (order), Glires, Euarchontoglires (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005173 stem cell factor receptor binding; GO:0030159 signaling receptor complex adaptor activity; GO:0035591 signaling adaptor activity; GO:0042301 phosphate ion binding; GO:0044877 protein-containing complex binding; GO:1990782 protein tyrosine kinase binding
  • GO BP:  GO:0001780 neutrophil homeostasis; GO:0007165 signal transduction; GO:0008285 negative regulation of cell population proliferation; GO:0030097 hemopoiesis; GO:0035162 embryonic hemopoiesis; GO:0035556 intracellular signal transduction; GO:0035702 monocyte homeostasis; GO:0035855 megakaryocyte development; GO:0036016 cellular response to interleukin-3; GO:0038163 thrombopoietin-mediated signaling pathway; GO:0043409 negative regulation of MAPK cascade; GO:0045589 regulation of regulatory T cell differentiation; GO:0046426 negative regulation of receptor signaling pathway via JAK-STAT; GO:0048821 erythrocyte development; GO:0051898 negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; GO:0060218 hematopoietic stem cell differentiation; GO:0060761 negative regulation of response to cytokine stimulus; GO:0070100 negative regulation of chemokine-mediated signaling pathway; GO:0090331 negative regulation of platelet aggregation; GO:1900235 negative regulation of Kit signaling pathway; GO:1903671 negative regulation of sprouting angiogenesis; GO:1904893 negative regulation of receptor signaling pathway via STAT; GO:1990869 cellular response to chemokine
  • GO CC:  NA

Sequence Information

  • Sequence:  YPWF
  • Length:  4
  • Propeptide:  MAGRTQGKHRPRVRTCPSLVPFDNPCWPPSHQPLPLQVGAPGHRVTSFLSGRAWPSYIPKGKHGLPGPRYVPDPWSLGVLGHVAPLRVTTTIFLPGASPGVMLDPACQKTDHFLSCYPWFHGPISRVRAAQLVQLQGPDAHGVFLVRQSESRRGEYVLTFNLQGRAKHLRLVLTERGQCRVQHLHFPSVVDMLRHFQRSPIPLECGAACDVRLSGYVVVVSQAPGRKPKVVSRGGVTASTARTKLPSWLRLLHGVIHVISPGSSNTVLFPFSLPHWDSELGHPHLSSAGCPPGHGAEALRGQVTPPEQIFHLVPSPEELANSLRQLELESVSSARDSDYEMDSSSRSHLRAIDNQYTPLSQLCREANL
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Elicit analgesia;decreased the systemic arterial pressure (SAP) and heart rate (HR) through a naloxone-sensitive mechanism
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-P50745-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor003233_AF2.pdbhor003233_ESM.pdb

Physical Information

Mass: 66538 Formula: C34H37N5O6
Absent amino acids: ACDEGHIKLMNQRSTV Common amino acids: FPWY
pI: 6.09 Basic residues: 0
Polar residues: 1 Hydrophobic residues: 2
Hydrophobicity: -25 Boman Index: 517
Half-Life: 2.8 hour Half-Life Yeast: 10 min
Half-Life E.Coli: 2 min Aliphatic Index 0
Instability Index: 3115 Extinction Coefficient cystines: 6990
Absorbance 280nm: 2330

Literature

  • PubMed ID:  18433935
  • Title:  Differential Cardiovascular Effects of Synthetic Peptides Derived From endomorphin-1 in Anesthetized Rats