General Information

  • ID:  hor002952
  • Uniprot ID:  P86130
  • Protein name:  signiferin 1
  • Gene name:  NA
  • Organism:  Crinia signifera (Common eastern froglet)
  • Family:  NA
  • Source:  animal
  • Expression:  Expressed by the skin glands.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Crinia (genus), Myobatrachinae (subfamily), Myobatrachidae (family), Myobatrachoidea (superfamily), Neobatrachia (suborder), Anura (order), Batrachia (superorder), Amphibia (class), Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0008083 growth factor activity
  • GO BP:  GO:0006952 defense response; GO:0007165 signal transduction; GO:0042311 vasodilation; GO:0045987 positive regulation of smooth muscle contraction; GO:0070665 positive regulation of leukocyte proliferation; GO:0097746 blood vessel diameter maintenance
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  RLCIPYIIPC
  • Length:  10(1-10)
  • Propeptide:  RLCIPYIIPC
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Induces mouse splenocyte proliferation and guinea pig smooth muscle contraction through binding to CCK-BR
  • Mechanism:  NA
  • Cross BBB:  NO
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  45726
  • Structure ID:  AF-O49519-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-O49519-F1.pdbhor002952_AF2.pdbhor002952_ESM.pdb

Physical Information

Mass: 135125 Formula: C55H91N13O12S2
Absent amino acids: ADEFGHKMNQSTVW Common amino acids: I
pI: 8.22 Basic residues: 1
Polar residues: 3 Hydrophobic residues: 4
Hydrophobicity: 133 Boman Index: 718
Half-Life: 1 hour Half-Life Yeast: 2 min
Half-Life E.Coli: 2 min Aliphatic Index 156
Instability Index: -430 Extinction Coefficient cystines: 1615
Absorbance 280nm: 179.44

Literature

  • PubMed ID:  16470724
  • Title:  Host-defence Skin Peptides of the Australian Streambank Froglet Crinia Riparia: Isolation and Sequence Determination by Positive and Negative Ion Electrospray Mass Spectrometry.
  • PubMed ID:  15317042
  • Title:  Disulfide-containing peptides from the glandular skin secretions of froglets