General Information

  • ID:  hor002778
  • Uniprot ID:  P62329
  • Protein name:  TYB4 28-44
  • Gene name:  Tmsb4x
  • Organism:  Rattus norvegicus (Rat)
  • Family:  Thymosin beta family
  • Source:  Animal
  • Expression:  By NGF and fibroblast growth factors. |Originally found in thymus but it is widely distributed in many tissues.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Rattus (genus), Murinae (subfamily), Muridae (family), Muroidea, Myomorpha (suborder), Rodentia (order), Glires, Euarchontoglires (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0003779 actin binding; GO:0003785 actin monomer binding; GO:0019899 enzyme binding; GO:0140311 protein sequestering activity
  • GO BP:  GO:0001649 osteoblast differentiation; GO:0007015 actin filament organization; GO:0030334 regulation of cell migration; GO:0033209 tumor necrosis factor-mediated signaling pathway; GO:0042989 sequestering of actin monomers; GO:0043124 negative regulation of canonical NF-kappaB signal transduction; GO:0043536 positive regulation of blood vessel endothelial cell migration; GO:0050728 negative regulation of inflammatory response; GO:2001028 positive regulation of endothelial cell chemotaxis; GO:2001171 positive regulation of ATP biosynthetic process
  • GO CC:  GO:0005634 nucleus; GO:0005737 cytoplasm; GO:0005829 cytosol; GO:0005856 cytoskeleton

Sequence Information

  • Sequence:  PLPSKETIEQEKQAGES
  • Length:  17(28-44)
  • Propeptide:  MSDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES
  • Signal peptide:  NA
  • Modification:  T4 Phosphoserine;T5 N6-acetyllysine;T7 Phosphothreonine;T12 N6-acetyllysine
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization (By similarity).
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  Actb
  • Target Unid:  P60711
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-P62329-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor002778_AF2.pdbhor002778_ESM.pdb

Physical Information

Mass: 215659 Formula: C79H131N21O31
Absent amino acids: CDFHMNRVWY Common amino acids: E
pI: 4.19 Basic residues: 2
Polar residues: 4 Hydrophobic residues: 3
Hydrophobicity: -144.71 Boman Index: -4620
Half-Life: >20 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: ? Aliphatic Index 51.76
Instability Index: 10140 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  23410195
  • Title:  Neurotoxin-Induced Neuropeptide Perturbations in Striatum of Neonatal Rats