General Information

  • ID:  hor002691
  • Uniprot ID:  Q93409
  • Protein name:  NLP-8
  • Gene name:  nlp-8
  • Organism:  Caenorhabditis elegans
  • Family:  NA
  • Source:  Animal
  • Expression:  Expressed in the ALA neuron in L4 stage larvae.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Caenorhabditis (genus), Peloderinae (subfamily), Rhabditidae (family), Rhabditoidea (superfamily), Rhabditomorpha (infraorder), Rhabditina (suborder), Rhabditida (order), Chromadorea (class), Nematoda (phylum), Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  NA
  • GO BP:  GO:0007218 neuropeptide signaling pathway; GO:0030431 sleep
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  FDRYDDETAYGYGFDNHIF
  • Length:  19(65-83)
  • Propeptide:  MSQKLLPISPLQLLFLQCLLIGFTAAYPYLIFPASPSSGDSRRLVKRAFDRFDNSGVFSFGAKRFDRYDDETAYGYGFDNHIFKRSADPYRFMSVPTKKAFDRMDNSDFFGAKRKRSFDRMGGTEFGLMKRSAPESREQLINNLAESIITLRRAREAESSPESQRTIITYDD
  • Signal peptide:  MSQKLLPISPLQLLFLQCLLIGFTAA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Neuropeptide-like protein (PubMed:30229400). Plays a role in behaviors associated with a sleep-like state induced by stress (SIS), acting in concert with the FARP (FMRFamide related) peptides, flp-13 and flp-24 (PubMed:27546573).
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q93409-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor002691_AF2.pdbhor002691_ESM.pdb

Physical Information

Mass: 266667 Formula: C108H137N25O35
Absent amino acids: CKLMPQSVW Common amino acids: D
pI: 3.88 Basic residues: 2
Polar residues: 7 Hydrophobic residues: 5
Hydrophobicity: -102.11 Boman Index: -5335
Half-Life: 1.1 hour Half-Life Yeast: 3 min
Half-Life E.Coli: 2 min Aliphatic Index 25.79
Instability Index: 3671.05 Extinction Coefficient cystines: 4470
Absorbance 280nm: 248.33

Literature

  • PubMed ID:  17564681
  • Title:  Impaired processing of FLP and NLP peptides in carboxypeptidase E (EGL-21)-deficient Caenorhabditis elegans as analyzed by mass spectrometry.