General Information

  • ID:  hor002334
  • Uniprot ID:  P01360
  • Protein name:  Califin-A
  • Gene name:  NA
  • Organism:  Aplysia californica (California sea hare)
  • Family:  Molluscan ELH family
  • Source:  Animal
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Aplysia (genus), Aplysiidae (family), Aplysioidea (superfamily), Aplysiida (order), Tectipleura, Euthyneura, Heterobranchia (subclass), Gastropoda (class), Mollusca (phylum), Lophotrochozoa, Spiralia, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity
  • GO BP:  GO:0007218 neuropeptide signaling pathway
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  ISINQDLKAITDMLLTEQIQARRRCLDALRQRLLDLGKRDSDVSLFNGDLLPNGRCS
  • Length:  57(117-173)
  • Propeptide:  MKANTMFIILCLSLSTLCVSSQSTSVHGKIFVPNRAVKLSSDGNYPFDLSKEDGAQPYFMTPRLRFYPIGKRAAGEMEQSEGQNPETKSHSWRKRSVLTPSLSSLGESLESGISKRISINQDLKAITDMLLTEQIQARRRCLDALRQRLLDLGKRDSDVSLFNGDLLPNGRCS
  • Signal peptide:  MKANTMFIILCLSLSTLCVSS
  • Modification:  T36 Leucine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Control the physiological processes of egg-laying
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  25-56
  • Structure ID:  AF-P01360-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor002334_AF2.pdbhor002334_ESM.pdb

Physical Information

Mass: 746955 Formula: C274H469N87O87S3
Absent amino acids: HWY Common amino acids: L
pI: 8.23 Basic residues: 9
Polar residues: 14 Hydrophobic residues: 20
Hydrophobicity: -35.26 Boman Index: -15023
Half-Life: 20 hour Half-Life Yeast: 30 min
Half-Life E.Coli: >10 hour Aliphatic Index 112.98
Instability Index: 5309.82 Extinction Coefficient cystines: 125
Absorbance 280nm: 2.23

Literature

  • PubMed ID:  3753705
  • Title:  Isolation and Primary Structure of the Califins, Three Biologically Active Egg-Laying Hormone-Like Peptides From the Atrial Gland of Aplysia Californica