General Information

  • ID:  hor002289
  • Uniprot ID:  Q7TSB7
  • Protein name:  Metastin
  • Gene name:  KISS1
  • Organism:  Rattus norvegicus (Rat)
  • Family:  KISS1 family
  • Source:  animal
  • Expression:  Expression observed in trophoblast giant cells (TGCs), the placenta-derived cell lineage aligned at the boundary between the uterus and placenta, at 12.5 dpc. Expressions gradually decreased during placental maturation, and the signal is no more detectabl
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Rattus (genus), Murinae (subfamily), Muridae (family), Muroidea, Myomorpha (suborder), Rodentia (order), Glires, Euarchontoglires (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0001664 G protein-coupled receptor binding; GO:0031773 kisspeptin receptor binding
  • GO BP:  GO:0007186 G protein-coupled receptor signaling pathway; GO:0007200 phospholipase C-activating G protein-coupled receptor signaling pathway; GO:0007204 positive regulation of cytosolic calcium ion concentration; GO:0008285 negative regulation of cell population proliferation; GO:0030336 negative regulation of cell migration; GO:0032874 positive regulation of stress-activated MAPK cascade; GO:0033686 positive regulation of luteinizing hormone secretion; GO:0043410 positive regulation of MAPK cascade; GO:0046697 decidualization; GO:0050806 positive regulation of synaptic transmission; GO:0060112 generation of ovulation cycle rhythm; GO:0060124 positive regulation of growth hormone secretion
  • GO CC:  GO:0005576 extracellular region; GO:0005615 extracellular space; GO:0016324 apical plasma membrane; GO:0043025 neuronal cell body

Sequence Information

  • Sequence:  TSPCPPVENPTGHQRPPCATRSRLIPAPRGSVLVQREKDMSAYNWNSFGLRY
  • Length:  52
  • Propeptide:  MISLASWQLLLLLCVASFGEPLAKMAPVVNPEPTGQQSGPQELVNAWQKGPRYAESKPGAAGLRARRTSPCPPVENPTGHQRPPCATRSRLIPAPRGSVLVQREKDMSAYNWNSFGLRYGRRQVARAARG
  • Signal peptide:  MISLASWQLLLLLCVASFG
  • Modification:  T43 Phosphotyrosine;T52 Tyrosine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Induces marked gonadotropin release and ovulation
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  Kiss1r
  • Target Unid:  Q924U1
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  4-18
  • Structure ID:  AF-Q7TSB7-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor002289_AF2.pdbhor002289_ESM.pdb

Physical Information

Mass: 674944 Formula: C254H399N79O74S3
Absent amino acids: Common amino acids: P
pI: 10.04 Basic residues: 8
Polar residues: 18 Hydrophobic residues: 12
Hydrophobicity: -80.96 Boman Index: -12779
Half-Life: 7.2 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 52.5
Instability Index: 5852.5 Extinction Coefficient cystines: 8605
Absorbance 280nm: 168.73

Literature

  • PubMed ID:  15219839
  • Title:  Peripheral administration of metastin induces marked gonadotropin release and ovulation in the rat.