General Information

  • ID:  hor002275
  • Uniprot ID:  P01048
  • Protein name:  T-kinin
  • Gene name:  Map1
  • Organism:  Rattus norvegicus (Rat)
  • Family:  NA
  • Source:  Animal
  • Expression:  In response to an inflammatory stimulant. T-kininogen II synthesis is induced and the plasma concentration of T-kininogen I is raised.|Plasma.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Rattus (genus), Murinae (subfamily), Muridae (family), Muroidea, Myomorpha (suborder), Rodentia (order), Glires, Euarchontoglires (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0004869 cysteine-type endopeptidase inhibitor activity; GO:0030414 peptidase inhibitor activity
  • GO BP:  GO:0006953 acute-phase response; GO:0007204 positive regulation of cytosolic calcium ion concentration; GO:0030195 negative regulation of blood coagulation; GO:0042311 vasodilation; GO:0097746 blood vessel diameter maintenance
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  ISRPPGFSPFR
  • Length:  11(376-386)
  • Propeptide:  MKLITILLLCSRLLPSLAQEEGAQELNCNDETVFQAVDTALKKYNAELESGNQFVLYRVTEGTKKDGAETLYSFKYQIKEGNCSVQSGLTWQDCDFKDAEEAATGECTTTLGKKENKFSVATQICNITPGKGPKKTEEDLCVGCFQPIPMDSSDLKPVLKHAVEHFNNNTKHTHLFALREVKSAHSQVVAGMNYKIIYSIVQTNCSKEDFPSLREDCVPLPYGDHGECTGHTHVDIHNTIAGFSQSCDLYPGDDL
  • Signal peptide:  MKLITILLLCSRLLPSLA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  As precursor of the active peptide bradykinin they effect smooth muscle contraction, induction of hypotension and increase of vascular permeability;They play a role in blood coagulation by helping to position optimally prekallikrein and factor XI next to
  • Mechanism:  Rats express four types of kininogens: the classical HMW and LMW kininogens produced by alternative splicing of the same gene, and two additional LMW-like kininogens: T-I and T-II.
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q9QXU9-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-Q9QXU9-F1.pdbhor002275_AF2.pdbhor002275_ESM.pdb

Physical Information

Mass: 143898 Formula: C59H89N17O14
Absent amino acids: ACDEHKLMNQTVWY Common amino acids: P
pI: 12.5 Basic residues: 2
Polar residues: 3 Hydrophobic residues: 3
Hydrophobicity: -51.82 Boman Index: -2482
Half-Life: 20 hour Half-Life Yeast: 30 min
Half-Life E.Coli: >10 hour Aliphatic Index 35.45
Instability Index: 11907.27 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  3121623
  • Title:  Purification and Characterization of Rat T-kininogens Isolated From Plasma of Adjuvant-Treated Rats. Identification of Three Kinds of T-kininogens.
  • PubMed ID:  3335530
  • Title:  Purification and Characterization of Two Kinds of Low Molecular Weight Kininogens From Rat (Non-Inflamed)