General Information

  • ID:  hor002273
  • Uniprot ID:  P21146
  • Protein name:  Leucokinin-7
  • Gene name:  GRK2
  • Organism:  Bos taurus (Bovine)
  • Family:  Protein kinase superfamily, AGC Ser/Thr protein kinase family, GPRK subfamily
  • Source:  animal
  • Expression:  Ubiquitous; brain, spleen > heart, lung > kidney.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Bos (genus), Bovinae (subfamily), Bovidae (family), Pecora (infraorder), Ruminantia (suborder), Artiodactyla (order), Laurasiatheria (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0000166 nucleotide binding; GO:0001664 G protein-coupled receptor binding; GO:0004672 protein kinase activity; GO:0004674 protein serine/threonine kinase activity; GO:0004703 G protein-coupled receptor kinase activity; GO:0005515 protein binding; GO:0005524 ATP binding; GO:0016301 kinase activity; GO:0016740 transferase activity; GO:0031694 alpha-2A adrenergic receptor binding; GO:0031755 Edg-2 lysophosphatidic acid receptor binding; GO:0047696 beta-adrenergic receptor kinase activity
  • GO BP:  GO:0002026 regulation of the force of heart contraction; GO:0002029 desensitization of G protein-coupled receptor signaling pathway; GO:0002031 G protein-coupled receptor internalization; GO:0003108 negative regulation of the force of heart contraction by chemical signal; GO:0006468 protein phosphorylation; GO:0006886 intracellular protein transport; GO:0007165 signal transduction; GO:0007186 G protein-coupled receptor signaling pathway; GO:0007213 G protein-coupled acetylcholine receptor signaling pathway; GO:0007217 tachykinin receptor signaling pathway; GO:0007507 heart development; GO:0009966 regulation of signal transduction; GO:0016310 phosphorylation; GO:0018105 peptidyl-serine phosphorylation; GO:0019079 viral genome replication; GO:0031623 receptor internalization; GO:0045880 positive regulation of smoothened signaling pathway; GO:0045988 negative regulation of striated muscle contraction; GO:0046718 symbiont entry into host cell; GO:0050896 response to stimulus; GO:0060048 cardiac muscle contraction; GO:1901081 negative regulation of relaxation of smooth muscle; GO:1903566 positive regulation of protein localization to cilium
  • GO CC:  GO:0005737 cytoplasm; GO:0005829 cytosol; GO:0005886 plasma membrane; GO:0016020 membrane; GO:0042995 cell projection; GO:0045202 synapse; GO:0098793 presynapse; GO:0098794 postsynapse

Sequence Information

  • Sequence:  DPAFSSWG
  • Length:  8
  • Propeptide:  NA
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Stimulates contractile activity of cockroach protodeum
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-P01194-F1(AlphaFold_DB_ID)
  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
  •    AF-P01194-F1.pdbhor002273_AF2.pdbhor002273_ESM.pdb

Physical Information

Mass: 99093 Formula: C40H51N9O13
Absent amino acids: CEHIKLMNQRTVY Common amino acids: S
pI: 3.75 Basic residues: 0
Polar residues: 3 Hydrophobic residues: 3
Hydrophobicity: -42.5 Boman Index: -746
Half-Life: 1.1 hour Half-Life Yeast: 3 min
Half-Life E.Coli: >10 hour Aliphatic Index 12.5
Instability Index: 4393.75 Extinction Coefficient cystines: 5500
Absorbance 280nm: 785.71

Literature

  • PubMed ID:  NA
  • Title:  Isolation, primary structure and synthesis of leucokinins VII and VIII: the final members of this new family of cephalomyotropic peptides isolated from head extracts of Leucophaea maderae.