General Information

  • ID:  hor002259
  • Uniprot ID:  B3EWL0
  • Protein name:  Leucokinin-like peptide
  • Gene name:  NA
  • Organism:  Delia radicum (Cabbage root fly) (Anthomyia brassicae)
  • Family:  Kinin family
  • Source:  Animal
  • Expression:  Detected in larvae. |Expressed in the CNS but not in midgut, ring gland, thoracic perisympathetic organs (tPSO) or abdominal perisympathetic organs (aPSO) (at protein level).
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Delia (genus), Anthomyiinae (subfamily), Anthomyiidae (family), Muscoidea (superfamily), Calyptratae, Schizophora, Cyclorrhapha, Eremoneura, Muscomorpha (infraorder), Brachycera (suborder), Diptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  NA
  • GO BP:  GO:0007218 neuropeptide signaling pathway
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  NSVVLGKKQRFHSWG
  • Length:  15(1-15)
  • Propeptide:  NSVVLGKKQRFHSWG
  • Signal peptide:  NA
  • Modification:  T15 Glycine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Acts through intracellular calcium in Malpighian tubule stellate cells to raise chloride conductance.
  • Mechanism:  NA
  • Cross BBB:  NO
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  NA

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor002259_AF2.pdbhor002259_ESM.pdb

Physical Information

Mass: 199289 Formula: C79H123N25O20
Absent amino acids: ACDEIMPTY Common amino acids: GKSV
pI: 11.82 Basic residues: 4
Polar residues: 5 Hydrophobic residues: 5
Hydrophobicity: -72 Boman Index: -2947
Half-Life: 1.4 hour Half-Life Yeast: 3 min
Half-Life E.Coli: >10 hour Aliphatic Index 64.67
Instability Index: 1194.67 Extinction Coefficient cystines: 5500
Absorbance 280nm: 392.86

Literature

  • PubMed ID:  22848525
  • Title:  Peptidomics of the Agriculturally Damaging Larval Stage of the Cabbage Root Fly Delia Radicum (Diptera: Anthomyiidae)