General Information

  • ID:  hor001898
  • Uniprot ID:  P15438
  • Protein name:  Glucagon-like peptide 1
  • Gene name:  gcg
  • Organism:  Lithobates catesbeianus (American bullfrog) (Rana catesbeiana)
  • Family:  Glucagon family
  • Source:  Animal
  • Expression:  Produced in the A cells of the islets of Langerhans in response to a drop in blood sugar concentration.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Lithobates (genus), Ranidae (family), Ranoidea (superfamily), Neobatrachia (suborder), Anura (order), Batrachia (superorder), Amphibia (class), Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity
  • GO BP:  GO:0007165 signal transduction; GO:0050896 response to stimulus
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  HADGTFTSDMSSYLEEKAAKEFVDWLIKGRPK
  • Length:  32(39-70)
  • Propeptide:  HSQGTFTSDYSKYLDSRRAQDFVQWLMNSKRSGGISXXHADGTFTSDMSSYLEEKAAKEFVDWLIKGRPKHADGSFTSDFNKALDIKAAQEFLDWIINTPVKE
  • Signal peptide:  NA
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Promotes hydrolysis of glycogen and lipids, and raises the blood sugar level.
  • Mechanism:  X's in the sequence were included by homology with other species sequences.
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:   NA
  • IC50:  NA
  • EC50:  NA
  • ED50:  NA
  • Kd:  NA
  • Half life:  NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q8K4P2-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-Q8K4P2-F1.pdbhor001898_AF2.pdbhor001898_ESM.pdb

Physical Information

Mass: 421122 Formula: C164H250N42O51S
Absent amino acids: CNQ Common amino acids: K
pI: 5.68 Basic residues: 6
Polar residues: 8 Hydrophobic residues: 10
Hydrophobicity: -73.44 Boman Index: -6710
Half-Life / Aliphatic Index: 3.5 hour Aliphatic Index: 55
Instability Index: 3804.69 Extinction Coefficient cystines: 6990
Absorbance 280nm: 225.48

Literature

  • PubMed ID:  3260236
  • Title:  Isolation of peptide hormones from the pancreas of the bullfrog (Rana catesbeiana). Amino acid sequences of pancreatic polypeptide, oxyntomodulin, and two glucagon-like peptides.