General Information

  • ID:  hor001775
  • Uniprot ID:  Q9ESQ9
  • Protein name:  Neuropeptide NPVF
  • Gene name:  Npvf
  • Organism:  Rattus norvegicus (Rat)
  • Family:  FARP (FMRFamide related peptide) family
  • Source:  animal
  • Expression:  Isoform 1 is expressed at high levels in the hypothalamus and eye. Isoform 2 is specifically expressed in a region between the dorsomedial hypothalamic and ventromedial hypothalamic nuclei.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Rattus (genus), Murinae (subfamily), Muridae (family), Muroidea, Myomorpha (suborder), Rodentia (order), Glires, Euarchontoglires (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005102 signaling receptor binding
  • GO BP:  GO:0007218 neuropeptide signaling pathway; GO:0032277 negative regulation of gonadotropin secretion
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  ANMEAGTMSHFPSLPQRF
  • Length:  18(108-125)
  • Propeptide:  MEIISSKRFILLTLATSSFLTSNTLCSDELMMPHFHSKEGYGKYYQLRGIPKGVKERSVTFQELKDWGAKKDIKMSPAPANKVPHSAANLPLRFGRNIEDRRSPRARANMEAGTMSHFPSLPQRFGRTTARRITKTLAGLPQKSLHSLASSELLYAMTRQHQEIQSPGQEQPRKRVFTETDDAERKQEKIGNLQPVLQGAMKL
  • Signal peptide:  MEIISSKRFILLTLATSSFLTSNTLC
  • Modification:  T18 Phenylalanine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Inhibit forskolin-induced production of cAMP
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  Npffr1
  • Target Unid:  Q9EP86
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q09367-F1(AlphaFold_DB_ID)
  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
  •    AF-Q09367-F1.pdbhor001775_AF2.pdbhor001775_ESM.pdb

Physical Information

Mass: 232469 Formula: C88H133N25O26S2
Absent amino acids: CDIKVWY Common amino acids: AFMPS
pI: 7.55 Basic residues: 2
Polar residues: 5 Hydrophobic residues: 5
Hydrophobicity: -40.56 Boman Index: -2780
Half-Life: 4.4 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 32.78
Instability Index: 6617.78 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  11852091
  • Title:  A novel rat hypothalamic RFamide-related peptide identified by immunoaffinity chromatography and mass spectrometry.