General Information

  • ID:  hor001504
  • Uniprot ID:  Q23212
  • Protein name:  TPLGTMRF-amide
  • Gene name:  flp-3
  • Organism:  Caenorhabditis elegans
  • Family:  FARP (FMRFamide related peptide) family
  • Source:  Animal
  • Expression:  Expressed from the comma stage of embryogenesis, during all larval stages, and in adults. |Each flp gene is expressed in a distinct set of neurons. Flp-3 is expressed in the IL1 and PQR neurons.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Caenorhabditis (genus), Peloderinae (subfamily), Rhabditidae (family), Rhabditoidea (superfamily), Rhabditomorpha (infraorder), Rhabditina (suborder), Rhabditida (order), Chromadorea (class), Nematoda (phylum), Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  NA
  • GO BP:  GO:0007218 neuropeptide signaling pathway; GO:0043051 regulation of nematode pharyngeal pumping; GO:0045759 negative regulation of action potential
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  TPLGTMRF
  • Length:  8(75-82)
  • Propeptide:  MISPNHLILLFCVNCAFLVASDATPKRSPLGTMRFGKRAIADEMTFEEDGYYPSNVMWKRSTVDSSEPVIRDQRTPLGTMRFGKRSAEPFGTMRFGKRNPENDTPFGTMRFGKRASEDALFGTMRFGKREDGNAPFGTMKFGKREAEEPLGTMRFGKRSADDSAPFGTMRFGKRNPLGTMRFGK
  • Signal peptide:  MISPNHLILLFCVNCAFLVASDA
  • Modification:  T8 Phenylalanine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  FMRFamides and FMRFamide-like peptides are neuropeptides. SAEPFGTMRF-amide inhibits the activity of dissected pharyngeal myogenic muscle system.
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:   NA
  • IC50:  NA
  • EC50:  NA
  • ED50:  NA
  • Kd:  NA
  • Half life:  NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q23212-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor001504_AF2.pdbhor001504_ESM.pdb

Physical Information

Mass: 104714 Formula: C41H67N11O11S
Absent amino acids: ACDEHIKNQSVWY Common amino acids: T
pI: 10.55 Basic residues: 1
Polar residues: 3 Hydrophobic residues: 2
Hydrophobicity: 7.5 Boman Index: -887
Half-Life / Aliphatic Index: 7.2 hour Aliphatic Index: 48.75
Instability Index: -1128.75 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  16061202
  • Title:  Discovering Neuropeptides in Caenorhabditis Elegans by Two Dimensional Liquid Chromatography and Mass Spectrometry