General Information

  • ID:  hor000965
  • Uniprot ID:  O02686
  • Protein name:  Big gastrin
  • Gene name:  GAST
  • Organism:  Ovis aries (Sheep)
  • Family:  Gastrin/cholecystokinin family
  • Source:  Animal
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Ovis (genus), Caprinae (subfamily), Bovidae (family), Pecora (infraorder), Ruminantia (suborder), Artiodactyla (order), Laurasiatheria (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity
  • GO BP:  GO:0007165 signal transduction; GO:0032094 response to food
  • GO CC:  GO:0005576 extracellular region; GO:0005615 extracellular space

Sequence Information

  • Sequence:  QLGLQDPPHMVADLSKKQGPWVEEEEAAYGWMDF
  • Length:  34(59-92)
  • Propeptide:  MQRLCAHALILVLALAAFCEASWKPHSQLQDAPVAPGANKGQEPLRMDRLGPASHPRRQLGLQDPPHMVADLSKKQGPWVEEEEAAYGWMDFGRRSAEEGDQHP
  • Signal peptide:  MQRLCAHALILVLALAAFCEA
  • Modification:  T29 Sulfotyrosine;T34 Phenylalanine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  CCKBR
  • Target Unid:  W5QBM9
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-O02686-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor000965_AF2.pdbhor000965_ESM.pdb

Physical Information

Mass: 449217 Formula: C176H257N43O54S2
Absent amino acids: CINRT Common amino acids: E
pI: 3.96 Basic residues: 3
Polar residues: 5 Hydrophobic residues: 11
Hydrophobicity: -70.88 Boman Index: -4589
Half-Life: 0.8 hour Half-Life Yeast: 10 min
Half-Life E.Coli: >10 hour Aliphatic Index 60.29
Instability Index: 6639.12 Extinction Coefficient cystines: 12490
Absorbance 280nm: 378.48

Literature

  • PubMed ID:  5665711
  • Title:  Isolation, structure and synthesis of ovine and bovine gastrins.