General Information

  • ID:  hor000639
  • Uniprot ID:  Q9VVF7
  • Protein name:  Drostatin-B5
  • Gene name:  Mip
  • Organism:  Drosophila melanogaster (Fruit fly)
  • Family:  Allatostatin family
  • Source:  Animal
  • Expression:  Up-regulated by sleep deprivation. |Expressed throughout development, with higher expression in larvae than in embryos (at protein level) . |In larvae, strongly expressed in the midgut region before and in between the copper cells, and in a group of cells
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  melanogaster subgroup, melanogaster group, Sophophora (subgenus), Drosophila (genus), Drosophilini (tribe), Drosophilinae (subfamily), Drosophilidae (family), Ephydroidea (superfamily), Acalyptratae, Schizophora, Cyclorrhapha, Eremoneura, Muscomorpha (infraorder), Brachycera (suborder), Diptera (order), Endopterygota (cohort), Neoptera (infraclass), Pterygota (subclass), Dicondylia, Insecta (class), Hexapoda (subphylum), Pancrustacea, Mandibulata, Arthropoda (phylum), Panarthropoda, Ecdysozoa, Protostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0001664 G protein-coupled receptor binding; GO:0005102 signaling receptor binding; GO:0005184 neuropeptide hormone activity
  • GO BP:  GO:0007186 G protein-coupled receptor signaling pathway; GO:0007218 neuropeptide signaling pathway; GO:0045968 negative regulation of juvenile hormone biosynthetic process; GO:1903999 negative regulation of eating behavior
  • GO CC:  GO:0005576 extracellular region; GO:0005615 extracellular space

Sequence Information

  • Sequence:  DQWQKLHGGW
  • Length:  10(193-202)
  • Propeptide:  MAHTKTRRTYGFLMVLLILGSACGNLVASGSAGSPPSNEPGGGGLSEQVVLDQLSESDLYGNNKRAWQSLQSSWGKRSSSGDVSDPDIYMTGHFVPLVITDGTNTIDWDTFERLASGQSAQQQQQQPLQQQSQSGEDFDDLAGEPDVEKRAWKSMNVAWGKRRQAQGWNKFRGAWGKREPTWNNLKGMWGKRDQWQKLHGGWGKRSQLPSN
  • Signal peptide:  MAHTKTRRTYGFLMVLLILGSACG
  • Modification:  T10 Tryptophan amide
  • Glycosylation:  NA
  • Mutagenesis:  3-3W->A: Complete loss of activity; 10-10W->A: Complete loss of activity

Activity

  • Function:  Stabilizes sleep and maintains sleep homeostasis to inhibit the activity of wake-promoting circuits, such as those that involve the pigment dispersing factor (pdf) neurons. Regulated by the circadian clock network and pathways associated with a sleep home
  • Mechanism:  All five Drostatin-B peptides activate SPR with comparable efficiency, however Drostatin-B4 is a slightly more potent ligand.
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-Q9VVF7-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor000639_AF2.pdbhor000639_ESM.pdb

Physical Information

Mass: 141510 Formula: C58H79N17O15
Absent amino acids: ACEFIMNPRSTVY Common amino acids: GQW
pI: 7.55 Basic residues: 2
Polar residues: 2 Hydrophobic residues: 3
Hydrophobicity: -164 Boman Index: -1855
Half-Life: 1.1 hour Half-Life Yeast: 3 min
Half-Life E.Coli: >10 hour Aliphatic Index 39
Instability Index: 1482 Extinction Coefficient cystines: 11000
Absorbance 280nm: 1222.22

Literature

  • PubMed ID:  21214272
  • Title:  Peptidomics and peptide hormone processing in the Drosophila midgut.
  • PubMed ID:  12171930
  • Title:  Peptidomics of the larval Drosophila melanogaster central nervous system.